Note: In computational biology, N50 is statistics of a set of contig or scaffold lengths. The N50 is similar to a mean or median of lengths, but has greater weight given to the longer contigs. It is used widely in genome assembly, especially in reference to contig lengths within a draft assembly.
Blue line: Median
Red line: Mean
Explanation: Basecalled reads length represents distribution plot (distplot) to show the relationship between read density on y-axis and basecall length as a logarithmic scale on x-axis for all barcodes in FASTQ file.
Note:
- A distplot or distribution plot depicts the variation in the data distribution.
- A logarithmic scale (or log scale) is a way of displaying numerical data over a very wide range of values in a compact way.